Biotechnology Class XII Syllabus

Unit V: Protein and Gene Manipulation (40 Marks)

Chapter I: Recombinant DNA Technology

Introduction; Tool of rDNA Technology; Marketing Recombinant DNA; Introduction of Recombinant DNA into Host Cells; Identification of Recombinants; DNA Library; DNA Probes; Hybridization Techniques; Polymerase Chain Reaction (PCR); DNA Secquencing; Site-directed Mutagenesis

Chapter II: Genomics and Bioinformatics

Introduction; Genome Sequencing Projects; Gene Prediction and Counting; Genome Similarity, SNPs and Comparative Genomics; Functional Genomics; Proteomics; History of Bioinformatics; Sequences and Nomenclature; Information Sources; Analysis using Bioinformatics Tools

Chapter III: Proteing Structure and Engineering

Introduction to the World of Proteins; 3-D Shape of Proteins; Structure-Function Relationship in Proteins
Purification of Proteins; Characterization of Proteins; Protein based Products; Designing Proteins

Unit VI: Cell Culture Technology (30 Marks)

Chapter I: Microbial Culture and Applications

Introduction; Microbial Culture Techniques; Measurement and Kinetics of microbial Growth; Scale up of microbial process; Isolation of microbial products; Strain isolation and Improvement; Applications of microbial culture technology; Biosafety of genetically modified microbes

Chapter II: Plant Cell Culture and Applications

Introduction; Cell and Tissue Culture Techniques; Applications of Cell and Tissue Culture; Gene Transfer Methods in Plants; Transgenic Plants with Beneficial Traits; Biosafety in Plant Genetic Engineering

Chapter III: Animal Cell Culture and Applications

Introduction; Animal Cell Culture Techniques; Characterisation of Cell Lines; Scale-up of Animal Culture Process; Applications of Animal Cell Culture; Stem Cell Technology; Bio safety of genetically modified animals